diff --git a/nonebot/plugin/__init__.py b/nonebot/plugin/__init__.py
index 77d3118d..349961d1 100644
--- a/nonebot/plugin/__init__.py
+++ b/nonebot/plugin/__init__.py
@@ -36,12 +36,12 @@ FrontMatter:
 from itertools import chain
 from types import ModuleType
 from contextvars import ContextVar
-from typing import Set, Dict, List, Optional
+from typing import Set, Dict, List, Tuple, Optional
 
 _plugins: Dict[str, "Plugin"] = {}
 _managers: List["PluginManager"] = []
-_current_plugin: ContextVar[Optional["Plugin"]] = ContextVar(
-    "_current_plugin", default=None
+_current_plugin_chain: ContextVar[Tuple["Plugin", ...]] = ContextVar(
+    "_current_plugin_chain", default=tuple()
 )
 
 
diff --git a/nonebot/plugin/export.py b/nonebot/plugin/export.py
index f27740ed..a64339f7 100644
--- a/nonebot/plugin/export.py
+++ b/nonebot/plugin/export.py
@@ -9,7 +9,7 @@ FrontMatter:
 
 import warnings
 
-from . import _current_plugin
+from . import _current_plugin_chain
 
 
 class Export(dict):
@@ -58,7 +58,7 @@ def export() -> Export:
         "See https://github.com/nonebot/nonebot2/issues/935.",
         DeprecationWarning,
     )
-    plugin = _current_plugin.get()
-    if not plugin:
+    plugins = _current_plugin_chain.get()
+    if not plugins:
         raise RuntimeError("Export outside of the plugin!")
-    return plugin.export
+    return plugins[-1].export
diff --git a/nonebot/plugin/manager.py b/nonebot/plugin/manager.py
index b136f27d..ece76620 100644
--- a/nonebot/plugin/manager.py
+++ b/nonebot/plugin/manager.py
@@ -24,7 +24,7 @@ from . import (
     _managers,
     _new_plugin,
     _revert_plugin,
-    _current_plugin,
+    _current_plugin_chain,
     _module_name_to_plugin_name,
 )
 
@@ -223,15 +223,15 @@ class PluginLoader(SourceFileLoader):
         setattr(module, "__plugin__", plugin)
 
         # detect parent plugin before entering current plugin context
-        parent_plugin = _current_plugin.get()
-        if parent_plugin and _managers.index(parent_plugin.manager) < _managers.index(
-            self.manager
-        ):
-            plugin.parent_plugin = parent_plugin
-            parent_plugin.sub_plugins.add(plugin)
+        parent_plugins = _current_plugin_chain.get()
+        for pre_plugin in reversed(parent_plugins):
+            if _managers.index(pre_plugin.manager) < _managers.index(self.manager):
+                plugin.parent_plugin = pre_plugin
+                pre_plugin.sub_plugins.add(plugin)
+                break
 
         # enter plugin context
-        _plugin_token = _current_plugin.set(plugin)
+        _plugin_token = _current_plugin_chain.set(parent_plugins + (plugin,))
 
         try:
             super().exec_module(module)
@@ -240,7 +240,7 @@ class PluginLoader(SourceFileLoader):
             raise
         finally:
             # leave plugin context
-            _current_plugin.reset(_plugin_token)
+            _current_plugin_chain.reset(_plugin_token)
 
         # get plugin metadata
         metadata: Optional[PluginMetadata] = getattr(module, "__plugin_meta__", None)
diff --git a/nonebot/plugin/on.py b/nonebot/plugin/on.py
index 54a15078..390d7a27 100644
--- a/nonebot/plugin/on.py
+++ b/nonebot/plugin/on.py
@@ -26,14 +26,14 @@ from nonebot.rule import (
     shell_command,
 )
 
-from .manager import _current_plugin
+from .manager import _current_plugin_chain
 
 
 def _store_matcher(matcher: Type[Matcher]) -> None:
-    plugin = _current_plugin.get()
+    plugins = _current_plugin_chain.get()
     # only store the matcher defined in the plugin
-    if plugin:
-        plugin.matcher.add(matcher)
+    if plugins:
+        plugins[-1].matcher.add(matcher)
 
 
 def _get_matcher_module(depth: int = 1) -> Optional[ModuleType]:
@@ -70,6 +70,7 @@ def on(
         block: 是否阻止事件向更低优先级传递
         state: 默认 state
     """
+    plugin_chain = _current_plugin_chain.get()
     matcher = Matcher.new(
         type,
         Rule() & rule,
@@ -79,7 +80,7 @@ def on(
         priority=priority,
         block=block,
         handlers=handlers,
-        plugin=_current_plugin.get(),
+        plugin=plugin_chain[-1] if plugin_chain else None,
         module=_get_matcher_module(_depth + 1),
         default_state=state,
     )
@@ -109,6 +110,7 @@ def on_metaevent(
         block: 是否阻止事件向更低优先级传递
         state: 默认 state
     """
+    plugin_chain = _current_plugin_chain.get()
     matcher = Matcher.new(
         "meta_event",
         Rule() & rule,
@@ -118,7 +120,7 @@ def on_metaevent(
         priority=priority,
         block=block,
         handlers=handlers,
-        plugin=_current_plugin.get(),
+        plugin=plugin_chain[-1] if plugin_chain else None,
         module=_get_matcher_module(_depth + 1),
         default_state=state,
     )
@@ -150,6 +152,7 @@ def on_message(
         block: 是否阻止事件向更低优先级传递
         state: 默认 state
     """
+    plugin_chain = _current_plugin_chain.get()
     matcher = Matcher.new(
         "message",
         Rule() & rule,
@@ -159,7 +162,7 @@ def on_message(
         priority=priority,
         block=block,
         handlers=handlers,
-        plugin=_current_plugin.get(),
+        plugin=plugin_chain[-1] if plugin_chain else None,
         module=_get_matcher_module(_depth + 1),
         default_state=state,
     )
@@ -189,6 +192,7 @@ def on_notice(
         block: 是否阻止事件向更低优先级传递
         state: 默认 state
     """
+    plugin_chain = _current_plugin_chain.get()
     matcher = Matcher.new(
         "notice",
         Rule() & rule,
@@ -198,7 +202,7 @@ def on_notice(
         priority=priority,
         block=block,
         handlers=handlers,
-        plugin=_current_plugin.get(),
+        plugin=plugin_chain[-1] if plugin_chain else None,
         module=_get_matcher_module(_depth + 1),
         default_state=state,
     )
@@ -228,6 +232,7 @@ def on_request(
         block: 是否阻止事件向更低优先级传递
         state: 默认 state
     """
+    plugin_chain = _current_plugin_chain.get()
     matcher = Matcher.new(
         "request",
         Rule() & rule,
@@ -237,7 +242,7 @@ def on_request(
         priority=priority,
         block=block,
         handlers=handlers,
-        plugin=_current_plugin.get(),
+        plugin=plugin_chain[-1] if plugin_chain else None,
         module=_get_matcher_module(_depth + 1),
         default_state=state,
     )
diff --git a/tests/plugins/nested/__init__.py b/tests/plugins/nested/__init__.py
index eb4cf3e4..6f514513 100644
--- a/tests/plugins/nested/__init__.py
+++ b/tests/plugins/nested/__init__.py
@@ -1,6 +1,13 @@
 from pathlib import Path
 
 import nonebot
+from nonebot.plugin import PluginManager, _managers
 
-_sub_plugins = set()
-_sub_plugins |= nonebot.load_plugins(str((Path(__file__).parent / "plugins").resolve()))
+manager = PluginManager(
+    search_path=[str((Path(__file__).parent / "plugins").resolve())]
+)
+_managers.append(manager)
+
+# test load nested plugin with require
+manager.load_plugin("nested_subplugin")
+manager.load_plugin("nested_subplugin2")
diff --git a/tests/plugins/nested/plugins/nested_subplugin.py b/tests/plugins/nested/plugins/nested_subplugin.py
index e69de29b..8cae942f 100644
--- a/tests/plugins/nested/plugins/nested_subplugin.py
+++ b/tests/plugins/nested/plugins/nested_subplugin.py
@@ -0,0 +1 @@
+from .nested_subplugin2 import a
diff --git a/tests/plugins/nested/plugins/nested_subplugin2.py b/tests/plugins/nested/plugins/nested_subplugin2.py
new file mode 100644
index 00000000..cbf42e7a
--- /dev/null
+++ b/tests/plugins/nested/plugins/nested_subplugin2.py
@@ -0,0 +1 @@
+a = "required by another subplugin"
diff --git a/tests/test_plugin/test_load.py b/tests/test_plugin/test_load.py
index a99852ec..53b1d1df 100644
--- a/tests/test_plugin/test_load.py
+++ b/tests/test_plugin/test_load.py
@@ -38,6 +38,19 @@ async def test_load_plugin(app: App, load_plugin: Set["Plugin"]):
     assert nonebot.load_plugin("some_plugin_not_exist") is None
 
 
+@pytest.mark.asyncio
+async def test_load_nested_plugin(app: App, load_plugin: Set["Plugin"]):
+    import nonebot
+
+    parent_plugin = nonebot.get_plugin("nested")
+    sub_plugin = nonebot.get_plugin("nested_subplugin")
+    sub_plugin2 = nonebot.get_plugin("nested_subplugin2")
+    assert parent_plugin and sub_plugin and sub_plugin2
+    assert sub_plugin.parent_plugin is parent_plugin
+    assert sub_plugin2.parent_plugin is parent_plugin
+    assert parent_plugin.sub_plugins == {sub_plugin, sub_plugin2}
+
+
 @pytest.mark.asyncio
 async def test_bad_plugin(app: App):
     import nonebot